locus

BSU39270

BSU_39270

outlinks

bsu

BSU39270

BSU_39270

The protein

Catalyzed reaction/ biological activity

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

Biological materials

Mutant

GP475 (erm), available in [SW|Jörg Stülke]'s lab

BKE39270 ([[gene|bglP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA

BKK39270 ([[gene|bglP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA

GP475 (erm), available in [SW|Jörg Stülke]'s lab [pubmed|23475962]

BKE39270 ([[gene|bglP]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA

BKK39270 ([[gene|bglP]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39270 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGTATACCTCCTTTTT, downstream forward: _UP4_TGAAAAAACTAATGGGGTGA

Biological materials

lacZ fusion

pGP600 (in [[protein|pAC6]]), available in [SW|Jörg Stülke]'s lab

pGP600 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab

The protein

[SW|Domains]

[SW|PTS EIIA domain] type-1 (aa 480-584) (according to UniProt)

[SW|PTS EIIB domain] type-1 (aa 1-86) (according to UniProt)

[SW|PTS EIIC domain] type-1 (aa 103-459) (according to UniProt)

Biological materials

GFP fusion

GP1266, [[gene|bglP]]-cfp, available in [SW|Jörg Stülke]'s lab [pubmed|23475962]